دسترسی یکساله به بیش از ۵۰۰ ژورنال روز جهان موجود در سامانه
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http://medilib.ir
- ﻣﺪﺕ ﺯﻣﺎﻥ : 365 ﺭﻭﺯ
قیمت : 3,800,000 تومان- قیمت ویژه : 1,900,000تومان
How will generative AI disrupt data science in drug discovery?
Jean-Philippe VertÂÂ
New precision-breeding law unlocks gene editing in England
Mario CaccamoÂÂ
How the Monty Hall problem is similar to the false discovery rate in high-throughput data analysis
Jingyi Jessica LiÂÂ
The global patent landscape of HER2-targeted biologics
Qingjian Li, Jiaqi Xu, Qianshu Sun, Zebang Zhang, Yuanjia Hu & Herui YaoÂ
Human gastrointestinal-tract variation catalogued
Jared B. FudgeÂÂ
Combining 47 human genomes into a single pangenome
Jared B. FudgeÂÂ
Electronic skin with onboard sensory feedback system
Jared B. FudgeÂ
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity
Multiplexed mapping of chromatin features at single-cell resolution
Single-cell multi-omics allows functional characterization of structural variants
The expanding vistas of spatial transcriptomics
Luyi Tian, Fei Chen & Evan Z. MacoskoÂ
doi : 10.1038/s41587-022-01448-2
The formation and maintenance of tissue integrity requires complex, coordinated activities by thousands of genes and their encoded products. Until recently, transcript levels could only be quantified for a few genes in tissues, but advances in DNA sequencing, oligonucleotide synthesis and fluorescence microscopy have enabled the invention of a suite of spatial transcriptomics technologies capable of measuring the expression of many, or all, genes in situ.
Identification of patient-specific CD4+ and CD8+ T cell neoantigens through HLA-unbiased genetic screens
Chiara M. Cattaneo, Thomas Battaglia, Jos Urbanus, Ziva Moravec, Rhianne Voogd, Rosa de Groot, Koen J. Hartemink, John B. A. G. Haanen, Emile E. Voest, Ton N. Schumacher & Wouter ScheperÂ
doi : 10.1038/s41587-022-01547-0
Cancer neoantigens that arise from tumor mutations are drivers of tumor-specific T cell responses, but identification of T cell-recognized neoantigens in individual patients is challenging. Previous methods have restricted antigen discovery to selected HLA alleles, thereby limiting the breadth of neoantigen repertoires that can be uncovered. Here, we develop a genetic neoantigen screening system that allows sensitive identification of CD4+ and CD8+ T cell-recognized neoantigens across patients’ complete HLA genotypes.
Integration of whole transcriptome spatial profiling with protein markers
Nir Ben-Chetrit, Xiang Niu, Ariel D. Swett, Jesus Sotelo, Maria S. Jiao, Caitlin M. Stewart, Catherine Potenski, Paulius Mielinis, Patrick Roelli, Marlon Stoeckius & Dan A. LandauÂ
doi : 10.1038/s41587-022-01536-3
Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling.
Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag
Marek Bartosovic & Gonçalo Castelo-BrancoÂ
doi : 10.1038/s41587-022-01535-4
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modalities at single-cell resolution using nanobody-Tn5 fusion proteins.
Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution
Tim Stuart, Stephanie Hao, Bingjie Zhang, Levan Mekerishvili, Dan A. Landau, Silas Maniatis, Rahul Satija & Ivan RaimondiÂ
doi : 10.1038/s41587-022-01588-5
Chromatin states are functionally defined by a complex combination of histone modifications, transcription factor binding, DNA accessibility and other factors. Current methods for defining chromatin states cannot measure more than one aspect in a single experiment at single-cell resolution.
scChIX-seq infers dynamic relationships between histone modifications in single cells
Jake Yeung, Maria Florescu, Peter Zeller, Buys Anton de Barbanson, Max D. Wellenstein & Alexander van OudenaardenÂ
doi : 10.1038/s41587-022-01560-3
Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression. Recent advances have enabled mapping of histone marks in single cells, but most methods are constrained to profile only one histone mark per cell.
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice
Carine Bouffi, Kathryn A. Wikenheiser-Brokamp, Praneet Chaturvedi, Nambirajan Sundaram, Gillian R. Goddard, Mark Wunderlich, Nicole E. Brown, Janet F. Staab, Rachel Latanich, Nicholas C. Zachos, Emily M. Holloway, Maxime M. Mahe, Holly M. Poling, Simon Vales, Garrett W. Fisher, Jason R. Spence, James C. Mulloy, Aaron M. Zorn, James M. Wells & Michael A. HelmrathÂ
doi : 10.1038/s41587-022-01558-x
Human intestinal organoids (HIOs) derived from pluripotent stem cells provide a valuable model for investigating human intestinal organogenesis and physiology, but they lack the immune components required to fully recapitulate the complexity of human intestinal biology and diseases.
Functional analysis of structural variants in single cells using Strand-seq
Hyobin Jeong, Karen Grimes, Kerstin K. Rauwolf, Peter-Martin Bruch, Tobias Rausch, Patrick Hasenfeld, Eva Benito, Tobias Roider, Radhakrishnan Sabarinathan, David Porubsky, Sophie A. Herbst, Büşra Erarslan-Uysal, Johann-Christoph Jann, Tobias Marschall, Daniel Nowak, Jean-Pierre Bourquin, Andreas E. Kulozik, Sascha Dietrich, Beat Bornhauser, Ashley D. Sanders & Jan O. KorbelÂ
doi : 10.1038/s41587-022-01551-4
Somatic structural variants (SVs) are widespread in cancer, but their impact on disease evolution is understudied due to a lack of methods to directly characterize their functional consequences.
A proteome-wide atlas of drug mechanism of action
Dylan C. Mitchell, Miljan Kuljanin, Jiaming Li, Jonathan G. Van Vranken, Nathan Bulloch, Devin K. Schweppe, Edward L. Huttlin & Steven P. GygiÂ
doi : 10.1038/s41587-022-01539-0
Defining the cellular response to pharmacological agents is critical for understanding the mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate-based high-throughput screening infrastructure for quantitative proteomics and profiled 875 compounds in a human cancer cell line with near-comprehensive proteome coverage.
Magnify is a universal molecular anchoring strategy for expansion microscopy
Aleksandra Klimas, Brendan R. Gallagher, Piyumi Wijesekara, Sinda Fekir, Emma F. DiBernardo, Zhangyu Cheng, Donna B. Stolz, Franca Cambi, Simon C. Watkins, Steven L. Brody, Amjad Horani, Alison L. Barth, Christopher I. Moore, Xi Ren & Yongxin ZhaoÂ
doi : 10.1038/s41587-022-01546-1
Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel.
Control-independent mosaic single nucleotide variant detection with DeepMosaic
Xiaoxu Yang, Xin Xu, Martin W. Breuss, Danny Antaki, Laurel L. Ball, Changuk Chung, Jiawei Shen, Chen Li, Renee D. George, Yifan Wang, Taejeong Bae, Yuhe Cheng, Alexej Abyzov, Liping Wei, Ludmil B. Alexandrov, Jonathan L. Sebat, NIMH Brain Somatic Mosaicism Network & Joseph G. GleesonÂ
doi : 10.1038/s41587-022-01559-w
Mosaic variants (MVs) reflect mutagenic processes during embryonic development and environmental exposure, accumulate with aging and underlie diseases such as cancer and autism. The detection of noncancer MVs has been computationally challenging due to the sparse representation of nonclonally expanded MVs.
What is hindering the drug development industry from achieving diversity, equity and inclusion in its workforce?
Jennifer Y. Kim & Emily BottoÂ
