A standard inoculum of the organism of interest is inoculated onto a Mueller-Hinton agar plate and incubated in the presence of a penicillin disk. After incubation, the zone edge of inhibition of growth around the penicillin disk is examined. (A) A fuzzy zone edge, or "beach," indicates the absence of a beta-lactamase. (B) A sharp zone edge, or "cliff," indicates the presence of a beta-lactamase.
Graphic 114731 Version 1.0
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