This model of the DQ7 HLA (peptide chains encoded by DQA1*05 and DQB1*03:01) shows the 45EV eplet in black. This has a corresponding Terasaki eplet (TerEp 2001) confirming its immunogenicity in vivo. The blue area on both the DQA and DQB chains surrounding the eplet represents the epitope. An antibody specific for the 45EV eplet will make contact with the amino acids contained in the larger blue region when it binds to the HLA at 45EV. This homology model was generated within Swissmodeller and considers the area for a structural epitope to be within 15 Å of the eplet. Resolved radiograph crystallography structures (2NNA, 1S9V, 402F, 5KSV, 4DBP) were used as templates (all templates had a resolution of 2.7 Å or less) for alignment of HLA allele sequence data (obtained from https://www.ebi.ac.uk/ipd/imgt/hla/) in Swissmodeller (https://www.swissmodel.expasy.org/); mean sequence homology was >95%, quality of the generated homology model was assessed by QMEAN and Ramachandran plots.
From: McCaughan JA, Tinckam KJ. Donor specific HLA antibodies & allograft injury: Mechanisms, methods of detection, manifestations and management. Transpl Int 2018; 31(10):1059-1070.
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