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Western blot interpretation for Lyme disease

Western blot interpretation for Lyme disease
Isotype Timing of appearance after exposure Bands to be considered
IgM First few weeks Two of the following: ospC (24), 39, 41
IgG After first few weeks Five of the following: 18, 23, 28, 30, 39, 41, 45, 58, 66, 93
Criteria for a positive Western blot (immunoblot) test. The Western Blot test should always be used in conjunction with a concomitant quantitative serologic test. A positive Western Blot test should not be used alone to confirm the diagnosis. Alternate criteria for IgM reactivity were proposed at a Centers for Disease Control and Prevention conference. Other points noted at that conference were the need for standardization of the antigen preparation and techniques used.
IgG: immunoglobulin G; IgM: immunoglobulin M.
Criteria derived from: Dressler F, Whalen JA, Reinhardt BN, Steere AC. Western blotting in the serodiagnosis of Lyme disease. J Infect Dis 1993; 167:392.
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