Criteria for Western blot diagnosis of Lyme disease

Type of infection	Isotype	Bands to be considered
First few weeks	IgM	Two of the following: ospC (24), 39, 41
After first few weeks	IgG	Five of the following: 18, 23, 28, 30, 39, 41, 45, 58,66, 93

Western_blot_diagnosis_of_L.htm

Criteria for positive Western blot (immunoblot) analysis in the serologic confirmation of infection with Borrelia burgdorferi (Lyme disease).

IgM: immunoglobulin M; IgG: immunoglobulin G.

Criteria derived from Dressler, F, Whalen, JA, Reinhardt BN, Steere AC, J Infect Dis 1993; 167:392. *Alternate criteria for IgM reactivity, proposed by a Centers for Disease Control and Prevention conference. Other points noted at that conference were the need for standardization of the antigen preparation and techniques used.

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Criteria for Western blot diagnosis of Lyme disease