Blood culture volumes in pediatric patients (blood culture set may use only one bottle)

Patient weight (kg)	Blood volume (mL) for first culture set	Blood volume (mL) for second culture set
≤1.0	2	–
1.1 to 2.0	2	2
2.1 to 12.7	4	2
12.8 to 36.3	10	10
>36.3	20 to 30	20 to 30

When ≤10 mL of blood is collected, it should be inoculated into a single aerobic blood culture bottle.

Adapted from: Miller JM, Binnicker MJ, Campbell S, et al. A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2018 update by the Infectious Diseases Society of America and the American Society for Microbiology. Clin Infect Dis 2018; 67:813.

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