In vivo screening protocol to detect the presence of aberrant adrenal hormone receptors in adrenal Cushing's syndrome

Time (min)	Day 1	Day 2	Day 3
–60	Fasting-supine	Fasting-supine	Fasting-supine
–15	*	*	*
0	Upright*	GnRH 100 microgram IV*	Glucagon 1 mg IV*
+30	Upright*	*	*
+60	Upright*	*	*
+90	Upright*	*	*
+120	Upright*	*	*
+150	Supine*	(Meal)
+180	Mixed meal*		Vasopressin 10 international units IM*^¶
+210	*		*
+240	*		*
+270	*	*	*
+300	*	TRH 200 microgram IV*^¶	*
+330		*
+360	ACTH 1-24 250 microgram IV*	*	Metoclopramide 10 mg orally*
+390	*	*	*
+420	*	*	*
+450	*		*
+480	*		*

GnRH: gonadotropin-releasing hormone; IV: intravenous; IM: intramuscular; TRH: thyrotropin-releasing hormone; ACTH: corticotropin.
* Blood samples for determination of cortisol, ACTH, other hormones, and vital signs.
¶ Availability of certain compounds varies between different countries. TRH not available in United States; vasopressin can be replaced by terlipressin 1 mg IV in some countries.

Reproduced with permission from: Lacroix A, Mircescu, Hamet P. Clinical evaluation of the presence of abnormal hormone receptors in adrenal Cushing's syndrome. The Endocrinologist 1999; 9:9. Copyright © 1999 Lippincott Williams & Wilkins.

Graphic 65003 Version 8.0

خرید پکیج

In vivo screening protocol to detect the presence of aberrant adrenal hormone receptors in adrenal Cushing's syndrome