Capillary electrophoresis of hemoglobin variants

Separation pattern and quantification of an artificial mixture of normal and some common variant hemoglobins by capillary electrophoresis (courtesy of Sebia®). The first of the two boxed items (top) is the reconstructed capillary electrophoretic separation pattern of the hemoglobins seen in the main electropherogram (main graph). The areas of the different Hb peaks (measured at 415 nm wavelength) are quantified and the relative percentage is calculated, as shown in the second (bottom) box.