72-hour fecal fat collection and calculation of coefficient of fat absorption

1. 48 to 72 hours prior to the beginning of stool collection, subject is started on a high-fat diet*.

2. All stools are collected into a single container for a full 72 hours. Stool container is kept refrigerated during collection.

3. During the collection, all food and fluid intake is recorded. A record of all bowel movements is kept. Intake record is analyzed for total dietary fat intake.

4. Collected stool is homogenized and analyzed for fat by the method of van de Kamer^[1]. MCT will not be measured by this method.

5. CFA is calculated using the following equation: CFA = (Dietary fat – Stool fat) ÷ Dietary fat

This protocol is intended for clinical use. This protocol is not sufficiently precise to be used for research purposes.

MCT: medium-chain triglyceride; CFA: coefficient of fat absorption.
* For children, a high-fat diet consists of >2 g/kg/day and a minimum of 50 g/day of fat. For adolescents and adults, the high-fat diet is 100 g/day of fat^[2]. MCT fat in the diet should not be counted towards this total fat intake (eg, from MCT supplements or formula).

Courtesy of Robert D Baker, MD.

Van de Kamer, JH, et al. Rapid method for the determination of fat in feces. J Biol Chem 1949; 177:347.
Taylor CJ, Chen K, Horvath K, et al. ESPGHAN and NASPGHAN Report on the Assessment of Exocrine Pancreatic Function and Pancreatitis in Children. J Pediatr Gastroenterol Nutr 2015; 61:144.

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72-hour fecal fat collection and calculation of coefficient of fat absorption