VWF multimer analysis

Agarose gel electrophoresis (1.0 percent) of plasma followed by fixation and exposure to a monospecific radiolabeled antibody to VWF. The highest molecular weight multimers of VWF are at the top of the gel (arrow). Lane 1 - severe vWD (type 3) plasma, lane 2 - normal pooled plasma, lane 3 - type 2A plasma, lane 4 - type 2B plasma, lane 5 - vWF extracted from platelets.