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Recommendations for safe laboratory practices to avoid exposure to Brucella spp

Recommendations for safe laboratory practices to avoid exposure to Brucella spp
When brucellosis is suspected, clinicians or forwarding laboratories should note on the laboratory submission: "Suspect or rule out brucellosis."
Review laboratory containment methods and microbiologic procedures to ensure compliance with recommendations in the Biosafety in Microbiological and Biomedical Laboratories, Fifth Edition.
Use primary barriers (ie, safety centrifuge cups, personal protective equipment, and class II or higher biological safety cabinets [BSCs]) for procedures with a high likelihood of producing droplet splashes or aerosols.
Use secondary barriers: Restrict access to the laboratory when work is being performed and maintain the integrity of the laboratory air-handling system by keeping external doors and windows closed.
Avoid causing splashes or aerosols when performing procedures on unidentified isolates.
Prohibit sniffing of open culture plates to assist in the identification of isolates.
Manipulate isolates of small gram-negative or gram-variable rods initially inside a BSC.
Data from: MMWR Surveill Summ 2008; 57:39.
Graphic 78971 Version 3.0

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