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تعداد آیتم قابل مشاهده باقیمانده : 3 مورد
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Molecular assays for rapid detection of MRSA

Molecular assays for rapid detection of MRSA
Assay Target(s)  Prevalence (%) Sensitivity (%) Specificity (%) Positive predictive value (%) Negative predictive value (%) Reference, year
Nose, groin, and throat assays
Xpert MRSA - third generation Staphylococcal protein A gene (spa), mecA, SCCmec-orfX junction 5.0 71.4 98.4 70.6 98.5 [1], 2019
Xpert MRSA - third generation Staphylococcal protein A gene (spa), mecA, mecC, SCCmec-orfX junction 4.7  82.3 96.8 55.7 99.1  [1], 2019
Xpert MRSA - third generation Staphylococcal protein A gene (spa), mecA, mecC, SCCmec-orfX junction 2.3 86.9 97.9 47.7 99.7 [2], 2017
Xpert MRSA - Next Generation Staphylococcal protein A gene (spa), mecA, mecC, SCCmec-orfX junction (incorporates new primer designs and other refinements) 4.3 84.3 99.1 81.4 99.3 [1], 2019
Xpert MRSA - Next Generation Staphylococcal protein A gene (spa), mecA, mecC, SCCmec-orfX junction (incorporates new primer designs and other refinements) 1.2  77.8 99.2  47.6 99.9 [2], 2017
BD MAX MRSA XT - third generation mecA, mecC, SCCmec-orfX junction 10 66.7 97.1 72.7 97.2 [3], 2015
LightCycler MRSA Advanced Test Insertion site SCCmec at orfX junction 6.8  98.3 98.9  86.7 99.9  [4], 2015
Blood assays
BD MAX Staph SR assay - second generation SCCmec right extremity junction, thermostable nuclease (nuc), mecA, mecC 10.4  100 100 100 100 [5], 2015
Xpert MRSA/SA BC - second generation Staphylococcal protein A gene (spa), mecA, SCCmec-orfX junction 7.3 100 100 100 100 [6], 2011
Xpert MRSA/SA SSTI - second generation Staphylococcal protein A gene (spa), mecA, SCCmec-orfX junction 14 98.3 99.4  96.6 99.7 [7], 2009
MRSA: methicillin-resistant Staphylococcus aureus; MSSA: methicillin-susceptible S. aureus; SSTI: skin and soft tissue infection.
References:
  1. Bulliard E, Grandbastien, Senn L, et al. Evaluation of three consecutive versions of a commercial rapid PCR test to screen for methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 2019.
  2. Jacqmin H, Schuermans A, Desmet S, et al. Performance of three generations of Xpert MRSA in routine practice: approaching the aim? Eur J Clin Microbiol Infect Dis 2017; 36:1363.
  3. Lepainteur M, Delattre S, Cozza S, Lawrence C, Roux AL, Rottman M. Comparative evaluation of two PCR-based methods for detection of methicillin-resistant Staphylococcus aureus (MRSA): Xpert MRSA Gen 3 and BD-Max MRSA XT. J Clin Microbiol 2015; 53:1955.
  4. Patel PA, Schora DM, Peterson KE, Grayes A, Boehm S, Peterson LR. Performance of the Cepheid Xpert SA nasal complete PCR assay compared to culture for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus colonization. Diagn Microbiol Infect Dis 2014; 80:32.
  5. Ellem JA, Olma T, O'Sullivan MV. Rapid detection of methicillin-resistant Staphylococcus aureus and methicillin-susceptible S. aureus directly from positive blood cultures by use of the BD Max StaphSR assay. J Clin Microbiol 2015; 53:3900.
  6. Spencer DH, Sellenriek P, Burnham CA. Validation and implementation of the GeneXpert MRSA/SA blood culture assay in a pediatric setting. Am J Clin Pathol 2011; 136:690.
  7. Wolk DM, Struelens MJ, Pancholi P, et al. Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays. J Clin Microbiol 2009; 47:823.
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