Survival by Asian Cancer Research Group (ACRG) gastric cancer subtype across multiple cohorts

(A) The ACRG molecular subtypes are associated with overall survival in the ACRG gastric cancer cohort. The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 107, n = 79, n = 68, and n = 46, respectively. Log-rank test showed an overall p = 4e to 4. The subtypes and associations with survival were replicated in three additional cohorts.
(B) SMC-2 (n = 277) cohort; GSE26253. The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 88, n = 85, n = 49, and n = 55, respectively. Cox trend test showed an overall p = 4e to 4.
(C) GSE15459, an independent cohort from Singapore (n = 200). The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 63, n = 39, n = 12, and n = 85, respectively. Cox trend test showed an overall p = 1e to 2.
(D) TCGA gastric cohort (n = 205). The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 47, n = 50, n = 46, and n = 62, respectively. Cox trend test overall p = 4e to 2.
(E) Merged SMC-2, Singapore, and TCGA cohorts. The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 198, n = 174, n = 107, and n = 202, respectively. Cox trend test showed overall p = 3e to 10, and log-rank test showed overall p = 1e to 8.
(F) Merged data for all four cohorts. The numbers of subjects in the MSS/TP53–, MSS/TP53+, MSI, and MSS/EMT subtypes are n = 305, n = 253, n = 175, and n = 248, respectively. Cox trend test overall p = 1e to 13, and log-rank test overall p = 6e to 12.