Test | Target of detection | Approximate time to positivity (days) |
Enzyme-linked immunoassay | ||
First generation | IgG antibody | 35 to 45 |
Second generation | IgG antibody | 25 to 35 |
Third generation | IgM and IgG antibody | 20 to 30 |
Fourth generation | IgM and IgG antibody and p24 antigen | 15 to 20 |
Western blot | ||
IgM and IgG antibody | 35 to 50 (indeterminate) | |
45 to 60 (positive) | ||
HIV viral load test | ||
Sensitivity cutoff 50 copies/mL | RNA | 10 to 15 |
Ultrasensitive cutoff 1 to 5 copies/mL | RNA | 5 |
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